Dear CCP4 community,
I have a question a bit off CCP4 topic, but that could use so expert input.
While discussing about a bacterial secreted hemophore (heme scavenging
protein) for which the apo form has been solved, it seems that attempts to
obtain the Heme bound form are failing; in fact during data collection on a
supposed Heme bound form, not only the Iron state changes (reduction), but
the Heme position seems to be affected too. Would anybody have some
experience with such a system and care to send some inputs (off line is fine
as we are here a bit off topic)? Thank you in advance for your help. Best
regards and Happy Building,
Steph
--
Stephane B. Richard, Ph.D., VP Business Development
MEDIT US, 7985 Dunbrook Rd., Ste A, San Diego, CA 92126, USA
MEDIT SA, 2 rue du Belvedere, 91120 Palaiseau, France
http://www.linkedin.com/in/stephanebrichard
Web: http://www.medit-pharma.com/
Email: [log in to unmask]
Cell: +1(858)342.6807
________________________________
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Nian
Huang
Sent: Monday, April 11, 2011 2:20 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] immobilized DNA resin
Heparin simulates the structure of DNA and RNA, so it has nonspecific
affinity towards DNA or RNA binding protein. It has also been used as DNAse
or RNase inhibitor but it is not very good one.
Nian Huang, Ph.D.
UT Southwestern Medical Center
On Sat, Apr 9, 2011 at 7:44 PM, Alexandra Deaconescu <[log in to unmask]>
wrote:
Hello ccp4 enthusiasts:
I am afraid this is a non-ccp4 related question. Can anyone
recommend an immobilized dsDNA chromatographic resin for purification of
DNA-binding proteins? GE seems to have something - I was wondering if people
have other recommendations? In the age of GST and His tags etc., these are
not very much used, but I do not have a tag in this case...
Thanks a lot,
Alex
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