We actually screen with unpurified oligos (up to just over 30nt) and it works just fine. Saves lots of time & $$.
If we get hopefull crystals, we pay for purification or do it ourselves by gel. Sometimes it makes the crystals better and sometimes it doesn't.
Phoebe
=====================================
Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.rsc.org/shop/books/2008/9780854042722.asp
---- Original message ----
>Date: Thu, 28 Apr 2011 08:32:23 -0500
>From: CCP4 bulletin board <[log in to unmask]> (on behalf of "Wallen, Jamie" <[log in to unmask]>)
>Subject: [ccp4bb] DNA oligonucleotide purification
>To: [log in to unmask]
>
> All,
>
> Our laboratory is looking for a new column to purify
> short (40mers or less) DNA oligonucleotides that
> will be used for crystallization setups. I wanted to
> ask if anyone has suggestions of a column that will
> give excellent separation of N and N-1 products. We
> have a Shimadzu HPLC system. We are considering
> Dionex columns as well as Oligonucleotide Separation
> C18 columns from Waters, but if there are others
> that are better we would like to know. Any
> suggestions will be greatly appreciated. Thank you!
>
> Jamie Wallen
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