Hello,
I have some problems with my polypeptide with two phosphorylated serine, an acetylated N-terminus and amidated C-terminus.
In a first time,
* I add the sequence:
Ace Pro Asp Sep Gly Ile Sep Leu Asn Thr Glu His Ser Val Glu Nh2
The error message is:
ApiError: No ChemCompVar found for protein:Ace linking start
Cannot find file:
Cannot find ChemComp XML file protein, Nh2.
ApiError: No ChemCompVar found for protein:Nh2 linking end
How define this modification and the link???
* In a second time, I try to build the polypeptide without the acetylated N-terminus and amidated C-terminus.
I add the sequence:Pro Asp Sep Gly Ile Sep Leu Asn Thr Glu His Ser Val Glu
If I look for the atom browser, for the assigment, I observed that the Sep have no HN. How to do the assignment of the HN of the Sep?
Could you explain me what is the Hua???
When I try to see the residue information, I obtained this error message:
Chemical component Sep available but no atom coordinates, so structure cannot be drawn.
Is this a significant problem for the future??
* After is it possible to use ARIA with a phosphorylated serine??
Thanks
Nathalie
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Nathalie Evrard-Todeschi
Equipe de RMN et Modélisation Moléculaire
Université Paris Descartes
Laboratoire de Chimie et Biochimie Pharmacologiques
et Toxicologiques. UMR 8601 CNRS.
45, rue des Saints-Pères,
75006 Paris
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