In addition to Bert remarks, you can read this paper from the positive
inside rule instigators.
Seppälä S, Slusky JS, Lloris-Garcerá P, Rapp M, von Heijne G. Control of
membrane protein topology by a single C-terminal residue. Science. 2010
Jun 25;328(5986):1698-700. Epub 2010 May 27. PubMed PMID: 20508091.
with the cited literature
Daniel
Le 04/03/2011 17:31, Justin Hall a écrit :
> Dear Community,
>
> In trying to trouble shoot an experiment I have become interested in the
> cellular process that regulates the insertion and proper orientation of
> membrane proteins. I am looking for references for how a GPCR is
> correctly oriented during expression (i.e. the extra cellular domain
> ends up extra cellularly oriented instead of a 50/50 mix in and out), my
> intuition is that there must be an N-terminal sequence that directs this
> process, but I am having no luck finding information on what this
> sequence is for GPCRs, what players are involved or how orientation is
> thought to be controlled. Any suggestions?
>
> This is all spurred by my wanting to use phage display with a protein
> that binds to the intracellular side of a GPCR, but of course that is
> the hard side to present to the outside of a cell so I need to figure
> out how to flip these guys around. I have thought about adding a new TM
> helix before TM1 (or removing TM1) to flip these guys, but was hoping
> there might be another way around that doesn't involve such massive
> architectural rearrangement such as simply clipping the N-terminal
> sequence responsible for proper orientation (if such a thing exists).
> Cheers~
>
> ~Justin
>
>
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