Hi Ray;
In case of my protein 5%-10% of glycerol help to increase the solubility
both of the soluble domain as well as the trans-membrane domain,improve
crystal quality dramatically and even prevent radiation damage to some
extent.
Best of luck
Bashir
On Thu, March 10, 2011 23:04, Ray Brown wrote:
> Hi all,
>
> I was intrigued by the recent question of whether glycerol had any adverse
> effects on the final purity of protein isolated by chromatography.
> Glycerol
> certainly helps to solubilize some proteins. Does anyone know of any
> negative
> effects of glycerol in protein purification, on protein crystal quality or
> use in cryocrystallography and on X-ray diffraction results?
>
> Cheers.
>
> Ray Brown
--
Muhammad Bashir Khan
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Department for Structural and Computational Biology
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