Dear all!
We've been trying to crystallize a helicase. No protein crystal was found after some typical conditions (kit I, kit II, and Index) were repeatly screened for more than three times. 50 mM MES (pH 6.0), 5% glycerol, and 1mM DTT is used as the final storage buffer. The most significant problem of this protein is its precipitation in most of the screening conditions. Protein concentration gradient was considered, but it can not work.
I was wondering if there is any way to work aroud this problem. Look forward to your suggestions!
Thanks!
Art
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