Hi Wataru:
I hope all is well. For the ones you already have grown, try very gently prying them off with a wedge-shaped needle.
If you can grow more, try using a very thin smooth layer of vacuum grease, and apply the drop to that. I managed to get RNA crystals to grow that way that otherwise irreversibly adhered to the surface.
All the best,
Bill
On Feb 8, 2011, at 6:06 PM, Wataru Kagawa wrote:
> Hi all,
>
> I have crystals growing by the hanging-drop method, using 24-well VDX plates and Hampton Research siliconized glass cover slides. Most crystals are attached to the cover slide, and I am having difficulties detaching the crystals (using a cryoloop) without breaking them. There are few smaller crystals floating in the drop, and they diffract X-rays pretty well (clean spots, ~3.5A resolution using RAXIS). However, I would like to try the bigger ones, because they may diffract to a higher resolution.
>
> Any suggestions for detaching crystals from cover slides will be greatly appreciated.
>
> Wataru
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