The way to go is make-your-own, especially since it is a pretty lousy enzyme (we often use 1:5 to 1:10 molar ratio of TEV:protein). You can get expression vectors here:
http://mcl1.ncifcrf.gov/waugh_tev.html
Bert
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From: CCP4 bulletin board [[log in to unmask]] On Behalf Of Gloria Borgstahl [[log in to unmask]]
Sent: Friday, January 14, 2011 9:36 AM
To: [log in to unmask]
Subject: [ccp4bb] TEV protease
We are using TEV protease to make a big batch of protein for
structural studies. We usually use thrombin, so this is our first
time using this enzyme on a large scale. Boy is it expensive! Does
anyone know of a bulk source for this enzyme and what ratios of use do
you recommend. Imidazole inhibits?
Many thanks. Gloria
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