It is normal for me, expressed a few proteins before and mass conformed all MET is SE-met. DQ
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From: CCP4 bulletin board [[log in to unmask]] On Behalf Of Yibin Lin [[log in to unmask]]
Sent: Thursday, January 06, 2011 3:06 PM
To: [log in to unmask]
Subject: [ccp4bb] selenomethinine-labeled protein
Dear all,
I try to express selenomethinine-labeled protein in E.coli methinoine auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18 hours at 37 degree. I don't why the colour of medium vary from colorless to yellow. Could anybody tell me it is normal or not?
Thanks a lot!
Lin
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