Quickly passing the crystal through Paratone N has worked well for me
when I crystallize in ammonium sulfate or sodium citrate conditions.
Another trick is to dissolve sucrose (table sugar) in 10uL of the
reservoir solution until it is saturated. Then separate the
sucrose-reservoir mix into two 5ul drops. Add 5ul of the reservoir
solution to one of the drops to make a 50% solution. Pass the crystal
through the 50% saturated sucrose solution, then the 100% saturated
sucrose solution and freeze. Good luck with the crystal!
Bryan
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-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Robert Kirchdoerfer
Sent: Wednesday, December 15, 2010 4:26 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] cryoprotectant for protein crystal grown from
Di-sodium hydrogen phosphate
I've had good luck cryoprotecting high salt crystal conditions with
sodium malonate (2.0-2.5M). Start with a 5M sodium malonate solution
and dilute to 40-50% with mother liquor.
good luck,
Rob
________________________________________
From: CCP4 bulletin board [[log in to unmask]] On Behalf Of Jerry
McCully [[log in to unmask]]
Sent: Wednesday, December 15, 2010 1:13 PM
To: [log in to unmask]
Subject: [ccp4bb] cryoprotectant for protein crystal grown from
Di-sodium hydrogen phosphate
Dear All;
Recently we got some crystals from the condition #51 in the SaltRx
crystallization kit from Hampton research.
It contains 1.5M Na2HPO4 and 0.1M Tris(pH8.5). We am going to do
a test diffraction ASAP.
What cryoprotectant did you use for this condition?
Thanks a lot and have a nice holiday season!
Jinghua
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