Is that necessary?
Eleanor
J. Preben Morth wrote:
> hi
> remember to reindex your data to P21212 in case you used Phaser to search all alternative orthorhombic SG's and it found P22121
> Preben
>
> On 03/10/2010, at 04.56, Jack Russel wrote:
>
>> Hi all,
>>
>> I have collected a data at 2.9 Å and the solved the structure using phaser . the space group comes to be P2 21 21. There are 4 molecules in Assymetric unit and an octamer is generated according to the symmetry. But after repeated rounds of rigid body refinement with REFMAC5 and model building with coot the R factor had been struck at 40% and R free at 50%.
>>
>> So my first question is whether my solution after phaser is correct. And if it is how can i lower the R factor and Rfree.
>>
>> The second question is it possible to have such a large difference between R factor and R free.
>>
>> Thanks in advance
>>
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