Hi Ed,
On Thu, Oct 21, 2010 at 02:19:34PM -0400, Ed Pozharski wrote:
> > * how the refinement in the lower-symmetry spacegroup is done - since
> > there is a real danger (in case it is the high-symmetry spacegroup
> > after all) that because of model bias and poorer (independent
> > observations)/parameter ratio what seems like a clear single
> > conformation is difficult to confirm.
>
> Absolutely true. As we discussed before, restraining protein copies is
> a must as well as maybe some bias removal.
As long as the bias removal isn't some brutal simulated-annealing job
that shakes everything about - and leads to those few significantly
differing sidechains that then convinces us that it really is a
lower-symmetry spacegroup.
Cheers
Clemens
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