How can you have 3 copies and one strong pseudo translation?You probably
need an even number of molecules with pairs related by the translation
vector.
Eleanor
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On 10/17/2010 05:48 AM, Mike John wrote:
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> Hi all,
> The data is 2.2A, p41212, unit cell has c axis twice longer
> than a or b. Data processed by HKL2000, the diffraction pattern shows
> Strong-weak-weak in C direction, indicating translational
> pseudo-symmetry.
> Xtriage analysis confirmed the pseudo-symmetry (large Patterson off-origin peak).
> The model is 100% identical searching prob.
> Phaser MR solved the structure easily with good Z-score and LLG(3 copies/a.u). The problem comes during the refinement:
> The R-free converged around 50% (R-factor 38%) and never go down even though I tried hard including, twin possibility.
> Seeking help to lower the R-free. Thank you very much
> Mike
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