Dear Xingliang,
This is vaguely reminiscent to a pattern that was observed by Zora
Markovic-Housley on precession picture of crystals of ornithine
aminotransferase: the even layer (l=2n) had a nice pattern diffracting
to high resolution while the odd layer (l=2n+1) had a honeycomb stucture
with the hole at the centre positioned were a diffraction spot was expected.
Reference:
Marković-Housley Z, Kania M, Lustig A, Vincent MG, Jansonius JN, John RA.
Eur J Biochem. 1987 Jan 15;162(2):345-50.
Quaternary structure of ornithine aminotransferase in solution and
preliminary crystallographic data.
Daniel
Le 14/09/2010 02:31, xingliang zhang a écrit :
> Dear everyone,
>
> Recently,we collected data of a native protein crystal on synchrotron
> in Shanghai. When we did with the original data with HKL2000, we found
> an unconversant phenomenon ,just as the picture in the enclosure, there
> were some grid indicated by arrows appearing on the low resolution
> areas.The crytal grew in 20%PEG3000,100mMTris-Cl,200mM Ca(OAc)2
> ,and the cryo-protectant is 20% sucrose mixed with well buffer。Who can
> tell me the reasons it appear the grid ?because of the sucrose? I’ll
> appreciated for any explains and suggestion.
>
> Best wishes
>
> xingliangzhang
>
>
>
> --
>
> Best wishes!
> xingliangcheung,PhD,candidate
>
> Protein crystallography Lab ,College of biological sciences ,China
> agricultural university.
>
> No. 2 yuanmingyuan west road HaidianDistrict, Beijing, 100193
>
> Tel:01062814122,
>
> E-mail:[log in to unmask] <mailto:[log in to unmask]>
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