Hi,
You can easily test the crystals by adding a drop of "Ponceau S"
staining solution. If you have protein crystals they will get stained.
Best wishes,
Tim
yybbll wrote:
> Hi,
> A little danger this condition. I suggest you should firstly make sure
> they are protein crystals. I know it is very difficult. I suggest you
> can add proteases to the drops. If "crystals" dissolve, no problem,
> they are protein crystals.
> Best
> Yibin
> 2010-08-27
> ------------------------------------------------------------------------
> yybbll
> ------------------------------------------------------------------------
> *发件人:* rui
> *发送时间:* 2010-08-27 02:04:54
> *收件人:* yybbll
> *抄送:*
> *主题:* Re: [ccp4bb] turn granular to crystal
> hi,
>
> no , I didn't use detergent. THe composition of the well buffer is
> just PEG4000 and MgCl2 and NaAc.
>
> On Wed, Aug 25, 2010 at 9:52 AM, yybbll <[log in to unmask]
> <mailto:[log in to unmask]>> wrote:
>
> Hi, Did you use detergent? It seems look like detergent crystals.
> 2010-08-25
> ------------------------------------------------------------------------
> yybbll
> ------------------------------------------------------------------------
> *发件人:* rui
> *发送时间:* 2010-08-25 20:37:18
> *收件人:* CCP4BB
> *抄送:*
> *主题:* [ccp4bb] turn granular to crystal
> Hi, All,
>
> I'm trying to crystallize a soluble protein and got something like
> granular, they are rounded shaped and not so regular and also
> don't have sharp edges. See the attached pic. The current
> condition is PEG4000 and pH around 5. How can I improve this
> condition? Thanks a lot.
>
>
>
--
Tim Schulte
Ruhr-Universita"t Bochum
LS Biophysik
AG Proteinkristallographie
Universita"tsstr. 150
44780 Bochum
Germany
Tel. +49-234-32-25754
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