Hi Lars
If I understand correctly, your query and reference are ok and you can
link from i to i-1 but not further. If this is the case, my first
suggestion would be to click the "goto i-1" button as this will then
find matches for the i-1 residue and then you can continue along the
sequence as far as your spectra will allow you to.
Hope this helps
Simon
Lars Backman wrote:
> Hi!
> Being new to ccpNMR I have run into a problem I do not know how to get around.
>
> I have used HNCA, HNCACB and CBCACONH experiments to pick peaks. This produces a peak list that seems to be alright.
> When opening Protein Sequence Assignment, selecting the only Query allowed (CBCACONH) and Match (HNCA and HNCACB), I can open the Spin System Table.
> The top part displays spin systems #1 ..... as well as spin systems at i-1 (these were picked and assigned from HNCA).
> Since the i-1 is already linked, I do not understand how to be able to link i-1 to i-2.
>
> I assume that I have made some error on the way to get here and appreciate any suggestions.
>
> lars
>
--
Simon P Skinner
Protein Chemistry Group
Leiden Institute of Chemistry, Universiteit Leiden
Phone: +31 71 527 6089 / Fax: +31 71 527 4349
E-mail : [log in to unmask]
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