Thurs., July 29th 2010
I think Andrew meant PDB 2gd1 and 1gd1 (has 4 NAD)
On Wed, 28 Jul 2010, A Leslie wrote:
> Hi Fred,
> If your tetramer show negative cooperativity between the 4 sites for
> ligand binding, then it is possible to get a tetramer with (say) only one
> subunit occupied by ligand, which can introduce considerable asymmetry if
> ligand binding gives rise to a hinge type closure of two domains around the
> ligand site. An example of this is glyceraldehyde 3-phosphate dehydrogenase
> which has been solved with one, two (and possibly 3) NADs bound per tetramer.
> I'm afraid you will need to do a literature search to find the references,
> but AJ Wonacott will be one of the authors.
> On 28 Jul 2010, at 19:31, Fred wrote:
>> Dear CCP4bb,
>> Could someone please, point me to some references about non-symmetric
>> tetramers? If I have a tetramer composed by 4 identical subunits, it'll
>> always have a P4 point group symmetry?
>> Thank in advance,