Hello,
The "Sequence Locations" table is a suggestion of where in the sequence
the selected spin system might belong, so in your case it thinks that spin
system {60} might be 187 Asp. That is based on how well the shifts within
the spin system match the chemical shifts in the RefDB database (I don't
know the detail, although Tim does). So it is not based on the current
assignment (the way I understand it). (Tim and Rasmus are in Portugal
this week giving a course so hard to ask them.)
If you don't want to use that prediction then you can just ignore it. (I
don't know how high the score has to be before one might believe it.) So
you can just use the "Set Seq Link" functionality in the middle table.
Wayne
On Tue, 1 Jun 2010, Marcel Jurk wrote:
> Hi everyone,
>
> I am, once again, encountering a strange problem during the course of
> assignment. Since this morning, analysis shows all sequence positions as
> already assigned in the "Sequence Locations" menu on the bottom left on the
> "Protein Sequence Assignment" panel (assign_locations.tiff), although none of
> these positions is actually assigned. Assignment status is displayed
> correctly under "Chart-->Assignment Graph" (see assign_graph.tiff).
> If a new spin system is assigned to one of the positions with misassigned
> status, analysis considers it indeed as assigned and asks for a merge of both
> spin systems (non-existing and new). Upon this, sequential links are
> disrupted regardless of choosing merge or no merge.
>
> Everything ran smoothly until today. I installed all the latest updates last
> Sunday but hadn't had a problem yesterday. Any suggestions as towards the
> root of this evil are very much appreciated.
>
> Many thanks in advance.
>
> Regards,
> Marcel
>
> --
> Marcel Jurk
>
> Leibniz Institut fuer Molekulare Pharmakologie (FMP)
> Solution NMR (AG Schmieder)
> Robert-Roessle-Str. 10
> 13125 Berlin
> Germany
>
> eMail: [log in to unmask]
> Phone: +49-30-94793223
> Fax: +49-030-94793169
>
>
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