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ACB-CLIN-CHEM-GEN  June 2010

ACB-CLIN-CHEM-GEN June 2010

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Subject:

Re: FW: CSF analysis

From:

ianholbrook <[log in to unmask]>

Reply-To:

ianholbrook <[log in to unmask]>

Date:

Thu, 24 Jun 2010 14:19:14 +0100

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (197 lines)

Nick
See
Clinical Chemistry 54: 930-932, 2008

http://www.clinchem.org/cgi/content/full/54/5/930


Kind regards
Ian
-----Original Message-----
From: Clinical biochemistry discussion list
[mailto:[log in to unmask]] On Behalf Of Nick Miller
Sent: 24 June 2010 14:09
To: [log in to unmask]
Subject: Re: FW: CSF analysis

I know this is a widely held view, but can anyone prove to me that fluoride
(by which we mean 5.0 mg of sodium fluoride in a maximum of 2.0 ml of
sample) "stops lactate production immediately"? (see below)

Nick Miller
London

On 24 June 2010 11:08, Holbrook, Ian B <[log in to unmask]> wrote:
>
> ________________________________
> From: Holbrook, Ian B
> Sent: 24 June 2010 11:07
> To: 'Turner Helen (NHS Grampian)'
> Cc: 'Dina'
> Subject: RE: CSF analysis
>
> Dear Helen
>
> On behalf of the UK Specialist Advisory Group for EQA of CSF Proteins
> and Biochemistry we would ALWAYS recommend analysing CSF if sufficient
> sample is received. This is precious material that often cannot be
repeated.
>
> If a blood stained CSF sample has a protein level within the reference
> range then this is useful information to the clinician. If it is above
> the reference range then of course appropriate comments must be added
> to help in interpretation. If the results could have been affected by
> the state of the CSF, delays in transport, not protected from the
> light etc. then comments on interpretation will form part of the report.
>
> I would think that you could analyse CSF glucose in an unpreserved
> sample as long as this was done within an hour of collection. Fluoride
> stops lactate production immediately but does not stop glucose
> utilisation for an hour or so. There would probably be little
> difference, therefore, between preserved and unpreserved samples within an
hour of collection.
>
> Kind regards
>
> Ian
>
>
> Ian Holbrook
>
> Department of Clinical Biochemistry
>
> York Hospital
>
> Wigginton Road
>
> York
>
> YO31 8HE
>
> 01904 725786
>
>
>
> ________________________________
> From: Clinical biochemistry discussion list
> [mailto:[log in to unmask]] On Behalf Of Turner Helen
> (NHS
> Grampian)
> Sent: 22 June 2010 17:52
> To: [log in to unmask]
> Subject: CSF analysis
>
> Dear readers,
>
> We are currently having a few disagreements regarding CSF analysis and
> hence I'm looking for some advice.
>
> 1- How to deal with blood stained samples for CSF protein, glucose,
> IgG, albumin and oligoclonal band analysis. Should these be reported
> as unsuitable, or analysed with a comment to say interpret with
> caution due to blood stained sample? In the case of oligoclonal bands-
> does the fact the sample is blood stained have an effect?
>
> 2- Tube type for CSF Glucose analysis. Does this have to be a Fl-Ox
> tube, or can a plain universal be used if received in the lab quickly
(<1hr).
>
>
> Thanks in advance
>
> Helen
>
> Clinical Biochemist
> Department of Clinical Biochemistry
> 1st Floor Link Building
> Aberdeen Royal Infirmary
> Foresterhill
> Aberdeen
> AB25 2ZD
>
> Tel: 01224 552831
>
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------ACB discussion List Information-------- This is an open discussion
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biochemistry.
Please note, archived messages are public and can be viewed via the
internet. Views expressed are those of the individual and they are
responsible for all message content.
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