The H+-ATPase from A. thaliana had anisotropic diffraction (ca.
3.6/5.5Å). This could be explained by the crystal packing and more
specifically the detergent micelles in the packing.
See:
Pedersen BP, Buch-Pedersen MJ, Morth JP, Palmgren MG, Nissen P. Crystal
structure of the plasma membrane proton pump. Nature 450, pp. 1111-4,
(2007).
Pedersen BP, Morth JP, Nissen P. Structure determination using poorly
diffracting membrane protein crystals - Lessons from the H+ and
Na+,K+-ATPases. Acta Cryst D, 66, pp. 309-313, (2010).
-Bjørn
On 2010-06-08 16:38, Matthias Zebisch wrote:
> Dear everybody!
>
> I am currently having an issue with anisotropic diffraction and would
> like to cite some references.
> I am sure there are plenty of examples outthere.
>
> So, if you are having an own published example at hand, can you please
> send me the reference and
> maybe the diffraction limits along good and bad directions as well?
>
> Thank you a lot,
>
> Matthias
>
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