Hi Murugan,
One useful indicator of raw anomalous signal is the ANOMPLOT graph
from Scala - this shows the differences between reflections compared
with the expected differences. If the gradient of the plot is 1
there's no more differences that you would expect. If the gradient is
more than one there is (or may be.) - also check out the merging
statistics as a function of batch, if there's significant radiation
damage this may mess things up.
Scala writes out the gradient (assuming you told it anomalous on) in the summary
Another rule-of-thumb is the resolution limit where cc-anom is > 0.5.
The most practical indicator of the anomalous signal is of course the
success or failure of the subsequent phasing :o)
Best wishes,
Graeme
On 29 June 2010 10:05, Vandu Murugan <[log in to unmask]> wrote:
> Dear all,
> I have collected a 2.7 angstrom home source data with Cu-Kalpha source
> for a protein with 6 cysteines, with a multiplicity of around 23. I need to
> know, is there any significant anamolous signal present in the data set,
> since there is no good model for my protein. Can any one tell, which
> program to run, and what parameter to see? Thanks in advance.
>
> cheers,
> Murugan
>
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