Colin
Fantastic discussion.
Well, answering your question, in my case (where we have about 5A difference
in cell length) the spots in the diffraction pattern look like “shooting
stars” (image attached).
I do not know exactly, but I believe that more than 300 crystals were
submitted to data collection. (By the way, of course I kept searching for a
different crystallization condition and I still do!!!). Anyway, I was looking
for crystals with a smaller twinning fraction and at the end the best data I
got display a 9.1% twinning fraction (image attached).
Even tough, the diffraction pattern improved I still had problem to process
the data. In my case, XDS program worked better than other programs. In
summary I used XDS to process my data set and phenix.striage to analyze the
twinning fraction and twin operator in order to solve this problem.
Cheers
Matheus
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Matheus Pinto Pinheiro
Laboratório de Cristalografia de Proteínas
Departamento de Física e Química
Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP
Fone: +55.16.3602.4193
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