I have no idea, it is not my buffer, it is dengzq1987's!
'BTW' means 'by the way', BTW.
>>> Enrico Stura <[log in to unmask]> 4/1/2010 11:01 am >>>
What is 1mM BTW? I am not familiar with this abbreviation.
PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc
BBS borate buffered saline (Borate + NaCl) wrong pH for zinc binding.
BTW ? (? ? ?) No idea what this is. Charles, do you know if it includes
HEPES or Phosphate?
On Thu, 01 Apr 2010 11:06:10 +0200, Charles Allerston
<[log in to unmask]> wrote:
> are you sure it is your protein precipitating? You will get a cloudy
> precipitate appearing in HEPES and Phosphate buffers on addition of
> ZnCl, without protein.
>>>> dengzq1987 <[log in to unmask]> 3/31/2010 5:08 pm >>>
> hello everyone, recently i purify a protein conteining zinc binding
> domain,and i want to determine its structure.i get the crystal,but poor
> diffraction.so i try to adding zinc into the protein to optimize the
> crystal,but the protein precipitate immidiately even the znic is 1
> mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does
> anyone have some advice?
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
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e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71