Hello,
I guess you mean the same spin system. (So the two amide H will have the
same N resonance but that will be different than the other resonances for
the spin system.) So there are several options here. If you don't know
anything else about the spin system (such as which residue it is) then you
could use the Resonance --> Spin Systems dialog and use the Merge button
at the bottom. But if you do assign them to the same residue then the
spin systems should automatically be merged.
But Initialise Root Resonances should also work assuming the peaks are not
already assigned (so if they are already assigned even with just anonymous
resonances then it won't work). So when you say nothing happened do you
mean that there were no Amide Side Chain Peaks listed or ...?
Wayne
On Mon, 29 Mar 2010, S.P. Skinner wrote:
> how do you make sure that they have the same n resonances as the backbone. I
> tried to intialise root resonances after starting assignment, but nothing
> happened. any ideas?
>
> Wayne Boucher wrote:
>> Hello,
>>
>> We're not quite sure we understand the question. So you can assign these
>> peaks in the normal way, only obviously make sure that they have the same N
>> resonance, and that the spin system is the same as for the backbone. But
>> possibly that's not what you are asking!
>>
>> Wayne
>>
>> On Mon, 29 Mar 2010, S.P. Skinner wrote:
>>
>>> Hey Guys
>>>
>>> Is there a way to include side chain amide peaks onto an HSQC once
>>> assignment has begun and approx 70% of the protein is assigned?
>>>
>>> Regards
>>>
>>> Simon
>>>
>>> --
>>> Simon P Skinner
>>> Protein Chemistry Group
>>> Leiden Institute of Chemistry, Universiteit Leiden
>>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>>> E-mail : [log in to unmask]
>
> --
> Simon P Skinner
> Protein Chemistry Group
> Leiden Institute of Chemistry, Universiteit Leiden
> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
> E-mail : [log in to unmask]
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