I assume the native and Se-met are isomorphous and indexed on the same
pOINTLESS will check the indexing convention for you.
Then you must use cad to merge the native and the phased data.
I am not sure of PHENIX output but you need an mtz file with
h k l Fnat etc F-Semet ... PHI FOM HLA etc
Then you just use parrot or DM to improve and extend the phases to the
limit of the native data.
In my experience it usually works brilliantly..
(reflection utilities) Jürgen Bosch wrote:
> DM ? Can you use NCS ?
> On Mar 7, 2010, at 6:43 PM, Stuart Endo-Streeter wrote:
>> Fellow crystallographers,
>> I am helping a college with a structure and we've run into a bit of a
>> jam. She has a 2.5 ang sel-met dataset, solved, and a 1.8 ang native
>> dataset. We would like to extend the phase solution from the anomalous
>> data to the native data. Unfortunately I have not been able to figure
>> out how to do this using CCP4. The phase solution was calculated using
>> PHENIX, which I have no real experience with yet, having worked with
>> SHELX, CNS, and CCP4 in the past. Can anyone point out the programs, if
>> any, in CCP4 (v6.1.3) to extend the phases from the 2.5 anomalous to the
>> 1.8 native data?
>> Stuart Endo-Streeter
> Jürgen Bosch
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