side chain amide peaks were listed, but when i hit the initalise peak
lists button, there was no list produced, probably cos all peaks had
been assigned to spin systems. I'll use the merge option and that
should overcome the problem.
Simon
Wayne Boucher wrote:
> Hello,
>
> I guess you mean the same spin system. (So the two amide H will have
> the same N resonance but that will be different than the other
> resonances for the spin system.) So there are several options here.
> If you don't know anything else about the spin system (such as which
> residue it is) then you could use the Resonance --> Spin Systems
> dialog and use the Merge button at the bottom. But if you do assign
> them to the same residue then the spin systems should automatically be
> merged.
>
> But Initialise Root Resonances should also work assuming the peaks are
> not already assigned (so if they are already assigned even with just
> anonymous resonances then it won't work). So when you say nothing
> happened do you mean that there were no Amide Side Chain Peaks listed
> or ...?
>
> Wayne
>
> On Mon, 29 Mar 2010, S.P. Skinner wrote:
>
>> how do you make sure that they have the same n resonances as the
>> backbone. I tried to intialise root resonances after starting
>> assignment, but nothing happened. any ideas?
>>
>> Wayne Boucher wrote:
>>> Hello,
>>>
>>> We're not quite sure we understand the question. So you can assign
>>> these peaks in the normal way, only obviously make sure that they
>>> have the same N resonance, and that the spin system is the same as
>>> for the backbone. But possibly that's not what you are asking!
>>>
>>> Wayne
>>>
>>> On Mon, 29 Mar 2010, S.P. Skinner wrote:
>>>
>>>> Hey Guys
>>>>
>>>> Is there a way to include side chain amide peaks onto an HSQC once
>>>> assignment has begun and approx 70% of the protein is assigned?
>>>>
>>>> Regards
>>>>
>>>> Simon
>>>>
>>>> --
>>>> Simon P Skinner
>>>> Protein Chemistry Group
>>>> Leiden Institute of Chemistry, Universiteit Leiden
>>>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>>>> E-mail : [log in to unmask]
>>
>> --
>> Simon P Skinner
>> Protein Chemistry Group
>> Leiden Institute of Chemistry, Universiteit Leiden
>> Phone: +31 71 527 6089 / Fax: +31 71 527 4349
>> E-mail : [log in to unmask]
--
Simon P Skinner
Protein Chemistry Group
Leiden Institute of Chemistry, Universiteit Leiden
Phone: +31 71 527 6089 / Fax: +31 71 527 4349
E-mail : [log in to unmask]
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