One thing I found with 2,3-Butanediol was for some crystallizations you can make it up a cryo solution with it at 12% and then put a thin layer it on top of the crystal droplet in a sitting drop well. Then you can loop the crystal and pull it up through the cryo layer - then out and to N2(l). Just how brief this is depends on your dexterity and also how well the crystal is engaged in the loop. My crystals were not very happy about the cryo but I could make it brief enough to get them out intact.
Good luck,
Martyn
Martyn Symmons
MBU Cambridge
----- Original Message ----
From: Sean Seaver <[log in to unmask]>
To: [log in to unmask]
Sent: Wednesday, 24 March, 2010 18:47:38
Subject: Re: [ccp4bb] 2,3-butanediol
Dear all,
I would like to know which is the more suitable method of using 2,3-Butanediol as cryoprotectant? Addition in to reservoir buffer or crystal soaking or both can be tried?
Cheers
Gauri
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I am sure both could be tried, but have found quite few papers mentioning brief soaking with 2,3-butanediol:
Purification, crystallization and X-ray diffraction analysis of the extracellular part of the human Fc receptor for IgA, Fc[alpha]RI (CD89)
http://scripts.iucr.org/cgi-bin/paper?cnor=bw5002
5-10 seconds
Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus
http://scripts.iucr.org/cgi-bin/paper?cnor=gr2202
described as 'briefly'
Crystallization and preliminary X-ray analysis of recombinant human acid [beta]-glucocerebrosidase, a treatment for Gaucher's disease
http://scripts.iucr.org/cgi-bin/paper?cnor=pu0086
described as 'briefly'
Crystallization and preliminary X-ray crystallographic studies of recombinant human betaine-homocysteine S-methyltransferase
http://scripts.iucr.org/cgi-bin/paper?cnor=gr2115
'quickly transferred'
Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus
http://scripts.iucr.org/cgi-bin/paper?cnor=pu0115
5% increments from 5 to 30% for 3 min at each step
Hope that helps,
Sean
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