Dear all --
I have a very "stupid" question/remark concerning this protein
concentration matter. Let say one has two different proteins, of 7 and
700 kDa, respectively. I guess that 20 mg/mL concentration would be
considered as a high concentration for the first protein, and probably
"less high" for the second. In order to be able to compare protocols,
is the notation mg/mL of proper choice? Would mM be a better option?
Or do we all assume working on the same averaged size sample?
Kind regards.
-- Leo --
made iPhone... Sorry for the eventual mistakes !
On 2010/03/06, at 2:44, "Murray, Michael (NIH/NIEHS)" <[log in to unmask]
> wrote:
> Jan,
>
> There is some good advice on dealing with proteins that do not
> self-aggregate here. Assuming that most or all of your drops are
> clear this
> might be useful.
>
> <http://web.emeraldbiosystems.com/blog/bid/24287/All-drops-clear-Back-to-Old
> -School-protein-crystallization>
>
> Good luck,
>
> Mike
>
> Michael S. Murray, Ph.D.
> National Institute of Environmental Health Sciences
> MD E3-01
> P.O. Box 12233
> Research Triangle Park, NC 27709
> Phone: (919) 541-0268
>
>
> On 3/5/10 9:02 AM, "Jan Rash" <[log in to unmask]> wrote:
>
>> Dear All,
>>
>> This is about the crystallization of the macromolecular complex
>> which is
>> highly soluble and shows no signs of the aggregation (even at high
>> concentration). We have tried several salts, precipitants and even
>> high
>> protein concentration (around 20g/L) for its crystallization
>> without any
>> genuine hit. Any suggestions for growing the crystals of this
>> macromolecular
>> complex will be highly appreciated.
>>
>> Thanks,
>>
>> Jan
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