Dear Lalti,
Apologies, but I am out of active NMR for a few years now, and the
difference between 'filtered' and 'edited' was never blindinhgly obvious.
Can you confirm that:
'Filtered' means that you see protons on *unlabelled* heavy atoms?
'Edited' means that you see protons on *labelled* heavy atoms?
This aside, we do not have a way to mark one chain as labeled, anothr as
unlabeled. It is on the todo list, but not yet. But we can at least get
the Experiment descriptions right.
Yours,
Rasmus
---------------------------------------------------------------------------
Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Mon, 25 Jan 2010, Lalit Deshmukh wrote:
> Hey Rasmus,
>
> Following Filtered NOESY experiments (source: Varian Biopack) are done on
> uniform 13C, 15N labeled protein+ non labeled peptide:
>
> a)gnoesyChsqc_CNfilt: F1 13C, 15N-filtered, F2 13C-edited NOESY-HSQC.
> Isotope filtering is done according to the scheme by Stuart et al, JACS,
> 121, 22, 5346-47, 1999.
>
> b)gnoesyNhsqc_CNfilt: F1 13C, 15N-filtered, F2 15N-edited NOESY-HSQC.
> Isotope filtering is done the same way.
>
> c, d) 2D-CNfiltocsy and 2D-CNfilnoesy: 2D-tocsy and 2D noesy (F1 13C,15N
> filtered, F2 13C,15N filtered) of bound peptide with suppression of signals
> from 15N,13C labeled protein. Based upon original pulse sequence
> noesyf1cnf2cn_h2o_pfg.c.
>
> Thanks in advance,
>
> Regards,
> Lalit
>
>
>
>
>> Dear Lalit,
>>
>> There are two issues here: How to describe the experiment types, and how
>> to describe the molecule labelling state. On the topic of the first, could
>> you describe exactly what each experiment does? What is on each axis,
>> what is covalently bound to what, and what the pulse sequence does (HSQC,
>> double-half finlter, ...)? I could make a good guess, but it is too easy
>> to make misunderstandings here.
>>
>> We shall be coming back about how to specify the labelling state.
>>
>> Yours,
>>
>> Rasmus
>>
>> ---------------------------------------------------------------------------
>> Dr. Rasmus H. Fogh Email: [log in to unmask]
>> Dept. of Biochemistry, University of Cambridge,
>> 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
>>
>> On Thu, 21 Jan 2010, Lalit Deshmukh wrote:
>>
>>> Hi,
>>>
>>> I am working with protein-peptide complex in Analysis. For the structure
>>> calculations of the complex, I have three sets of spectra collected on
>>> 13C,
>>> 15N labeled protein+ non-labeled peptide sample:
>>>
>>> a) 3D 15N-edited and 13C-edited NOESYs
>>> b) 2D and 3D 13C, 15N filtered-NOESYs
>>>
>>> When I define my Analysis project, I say Protein is chain A and Peptide
>>> is
>>> chain B. How to set up the Analysis project to distinguish between inter
>>> and
>>> intramolecular NOEs for these edited and filtered NOESY spectra?
>>>
>>> Thanks in advance,
>>>
>>> Regards,
>>> Lalit
>>>
>>
>
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