Dear Subbu,
One more thing you can do is to search with an ensemble of structures (4
in your case) for molecular replacement.
Fred.
Narayanan Ramasubbu wrote:
> Deal All:
> I have a 2.0 A data for a SeMet protein (native crystal not available
> yet!) that has 6 Se sites.
> The cell comes out to be 65 67 101 and the angles are all very close
> to 90. The data set was collected in house with Cu 1.5418 A
>
> We integrated and scale in orthorhombic and the statistics are
> reasonable.
>
> There are 4 homologous structures and all of them have a sequence
> identity of 15-16 %.
>
> I have tried Phaser, AMoRe, EPMR with varying templates
> with polyala, polyser and varying the resolutions.
>
> I have also done a modeller and found best match with one of the four
> structures. I have used this derived model as well for input.
>
> I would like to know whether there other options (I am working on
> getting a good synchrotron data set at the peak wavelength for Se).
>
> Thanks
> Subbu
> PS: I am also trying P1 just in case.
>
>
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