Pankaj Chauhan wrote:
> Hi all,
> In one of my 2.52 A structure, which is dimeric, the R-factor is 21.4 and
> free-R is 29.3. I have tried all ways to reduce the free-R including TLS
> (even by defining the number of TLS group per chain), but still factors are
> not getting reduced. Even i have tried some other space group, but no use.
> Even i have the same structure with some complex at 1.86 A in the same space
> group , but everything is fine in that.
> Any suggestion,
It worries me to have these discussions again..
You might well expect that sort of difference between R and FreeR at
your resolution - there really is nothing to worry about there.. See the
Some crystals are more poorly ordered than others..