Artem has already responded - but I believe you will pull down the
cellular debris with the IB.
In my prior experience, post centrifugation, you can wash the IB/debris
with increasing amounts of urea in you solubilization - and you may find
that the other cellular debris stays in solution before the IB goes in.
(1M, 2M, 4M urea....) - and after recentrifugation, I found that does a
pretty good job of cleaning up the media.
Ezra
megha goyal wrote:
>
>
> Dear All,
>
> Our protein is expressed as inclusion bodies and I want to separate
> inclusion bodies from E.coli from the cellular debris after* *lysis of
> the cells by sonication.
>
> Can I do this by normal centrifugation? and if yes, at what speed?
>
> Our centrifuge has maximum speed of 14000 rpm. Can we do the
> separation using this centrifuge and if so how.
>
>
>
> Thanking in anticipation.
>
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