Hello all,
Anyone have any tips for reducing agents for use with the following
crystallization conditions:
100 mM hepes, pH 7.5, 24% PEG 1500
or
100 mM Tris, pH 8.0, 24% PEG 1500
I've tried BME and DTT (1 mM). Currently trying out TCEP. The protein
seems to be sensitive to oxidation, with a selenomet-derivative even more so.
Thanks!
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