Hi everyone,
I was also trying to use the "Protein Sequence Assignment" function, and I have come across the same problem as Vicky, and another problem as well. I was using a HNCACB as the query and a HNCOCACB as the match, and got the same error message. I did find however, that if I query or match more than one experiment at the same time, the error does not appear.
Also, I am trying to match the accompanying carbonyl data as well (HNCACO and HNCO), but for some reason the HNCACO experiment is not given as an option to either query or match in the settings tab of the Protein Sequence Assignment function (but the others all appear there). As far as I can tell all the parameters for this spectrum are the same as for the others, but is there something stupid I have overlooked? Or is there something else going on?
Any help you can give me is greatly appreciated,
Thanks,
Ruth
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From: CcpNmr software mailing list [[log in to unmask]] on behalf of Vicky Higman [[log in to unmask]]
Sent: Friday, 2 October 2009 7:45 PM
To: [log in to unmask]
Subject: protein seq assign problem
Hi,
I'am trying to use the "Protein Sequence Assignment" function in the
same way as I previously used the "Link Seq Spin System" one, but am
getting a pop-up box error saying "Neither query nor matches spectra
represent any i -> i-1 specific experiments" and it doesn't look
for/find any matches. No message in the terminal window.
Unfortunately, I really can't work out what the problem is - I am using
a CBCANH as my query and CBCACONH as the match spectrum and I am
reasonably confident that I have the correct experiment prototypes
attached to these (see attached figure) - I certainly couldn't find any
that fitted these expts any better.
Am I missing something really obvious? Is there a bug here?
Any help gratefully received.
Thanks,
Vicky
--
*********************************************************
Dr. Victoria A. Higman
Leibniz-Institut fuer Molekulare Pharmakologie (FMP)
NMR-Supported Structural Biology
Robert-Roessle-Str. 10
13125 Berlin
Germany
and
School of Chemistry
University of Bristol
Bristol BS8 1TS
United Kingdom
Phone: +44-117-95-46325
E-mail: [log in to unmask] (or [log in to unmask])
http://www.protein-nmr.org.uk
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