On Wed, 28 Oct 2009, Gitte Wolfsberg Haxholm wrote:
> I have created the second set of spin-systems from the HSQC via "Assign",
> then giving each peak in the HSQC two new resonances and then clicking "set
> same spin system". I then used "Assignment : Pick and assign from roots" to
> link these to the peaks in the backbone spectra. to do this I picked the
> peaks and used "Assign" to assign these peaks to the corresponding
> spin-system from the HSQC.
So the only thing missing that "initialise root resonances" does is assign
the H & N atom types, probably that is what you need.
> What happens if I rerun the "initialise root resonances", does it then
> change/delete all the work I have been doing? With the linking to roots and
> so on?
No, it's safe to use as it will only add spin systems to unassigned peaks.
So no need to run it after what you have done above.
Dr. Brian O. Smith ---------------------- B Smith at bio gla ac uk
Division of Molecular & Cellular Biology,
Faculty of Biomedical & Life Sciences,
Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
Tel: 0141 330 5167/6459/3089 Fax: 0141 330 4600
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