Radovan Spurny wrote:
> I would like to ask for recommendations and tips how to use lipids for
> crystallization of membrane proteins. Especially I am interested about
> the solubilization of lipids (E.coli lipids, POPC, POPE, POPG) and how
> to mix them with protein.
>
> Thanks a lot.
>
Have a look at:
http://www.ncbi.nlm.nih.gov/pubmed/12702760
A defined protein-detergent-lipid complex for crystallization of integral membrane
proteins: The cytochrome b6f complex of oxygenic photosynthesis.
Zhang H, Kurisu G, Smith JL, Cramer WA.
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5160-3. Epub 2003 Apr 17.
> ________________________________________
>
> Radovan Spurny, PhD
> Laboratory of Structural Neurobiology
> Division of Pharmacology
> Campus Gasthuisberg, ON1
> Herestraat 49, PB 601
> B-3000 Leuven
> Belgium
> e-mail: [log in to unmask]
> <mailto:[log in to unmask]>
>
> web: www.xtal.be <http://www.xtal.be/>
>
>
>
|