Just a small note of caution:
It's not a bit of vector-derived sequence is it? A bit of peptide
linker between the protein of interest and any purification tags
maybe? I know of at least one case where a piece of vector derived
sequence has been present in the crystal (and in fact been critical
for crystallisation).
But hopefully this is not the case and you have been fortunate enough
to pull out something physiologically interesting :)
Regards,
David.
2009/10/30 Fabien Bergeret <[log in to unmask]>:
> Hello
>
> We’re resolving a structure of a soluble protein and in the electronic
> density map (maximum resolution at 2.2Å), we observe a supplementary density
> that does not belong to the protein. This density is present in two
> different crystalline forms obtained in different crystallization
> conditions.
> This density could be represented by an oligopeptide ~10 residues long for
> which there is no ambiguity about its polarity. Furthermore, side chains are
> quite easily visible and a sequence can therefore be assigned.
> The deduced sequence doesn’t belong to the sequence of the protein of
> interest, meaning that the oligopeptide has been co-purified and
> co-crystallized.
>
> Has somebody met a similar situation? Could you please give us some advices
> in terms of refinement, validation, etc.?
>
> Thanks in advance
>
> Best regards
>
>
> Fabien
> PhD student
> [log in to unmask] <mailto:[log in to unmask]>
>
--
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Science knows it doesn't know everything...otherwise it would stop.
- Dara O'Briain
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David C. Briggs PhD
Father & Crystallographer
http://drdavidcbriggs.googlepages.com/home
Skype: DocDCB
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