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CCP4BB  September 2009

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Subject:

Re: Devices Suitable to Concentrate Protein Solutions in Liter Scale

From:

Pius Padayatti <[log in to unmask]>

Reply-To:

Pius Padayatti <[log in to unmask]>

Date:

Sun, 6 Sep 2009 22:36:00 -0400

Content-Type:

text/plain

Parts/Attachments:

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text/plain (91 lines)

may be diafiltration devices might work for you. the membranes are
kind of expensive but we had used this effectively to reduce the volume
of cell culture media where we had secreted proteins in large volumes
of culture media.

here is a link
http://www.spectrapor.com/lit/hfdial.pdf


padayatti
On Sun, Sep 6, 2009 at 10:16 PM, Wu, Zhongren<[log in to unmask]> wrote:
> If you want to just reduce the volume/concentrate proteins, you can use
> Millipore’s Stirred cell  (high-output stirred cell, 2,000 ml capacity).
>
>
>
> Zhongren Wu
>
>
>
>
>
> ________________________________
>
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Xuan
> Yang
> Sent: Thursday, September 03, 2009 5:05 AM
>
> To: [log in to unmask]
> Subject: Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in
> Liter Scale
>
>
>
> Dear Dr Ku,
>
>
>
> It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg
> and 1mM EDTA.
>
>
>
> And I want to try other affinity columns, just don't know what resin would
> be appropriate.
>
>
>
> Sincerely,
>
>
>
> Xuan Yang
>
>
>
> 2009/9/3 Shao-Yang Ku <[log in to unmask]>
>
> Can you put a 6His-tag on your protein and add some Ni-NTA beads to your
> solution to capture (and concentrate) the properly folded molecules?
>
> Quoting "Xuan Yang" <[log in to unmask]>:
>
> Dear All,
>
> I am working on protein refolding via dialysis in large volumn
> (typically 2~4 litters). It was problematic when I wanted to concentrate the
> solution to at least less than 500ml. If you know any device appropriate for
> such task, please help me out:)
>
> Thanks in advance!
>
> Sincerely,
>
> Xuan Yang
>
>
>
>



-- 
Pius S Padayatti
Scientist,
Polgenix, Inc.
11000 Cedar Ave, Suite 260
Cleveland, OH 44106
Phone: 216-658-4528
Fax: 216-658-4529

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