Reply-To: | | [log in to unmask][log in to unmask], 6 Sep 2009 22:12:12 -0500489_US-ASCII Hi,
Could you clarify your request a bit? Generally speaking if you allow the ectodomain to have a membrane anchoring segment it will 'compromise' solubility since the resulting protein most likely requires special treatment (detergents) in order to be soluble. There's also the matter of the secretion signal - depending on your expression system you may succeed using the original secretion signal, or you may have to engineer an entirely new one. [...]36_6Sep200922:12:[log in to unmask] |