Dear Marija
This can be done by Proton Induced X-ray Emission on a tiny sample
(0.2microl) of liquid protein to get the sulphur to selenium ratio and
thus work out the substitution of Selenium.
See:
http://www.ionbeamcentre.co.uk/index.php?target=3:68
and contact
Dr. Chris Jeynes for this service:
[log in to unmask]
Best wishes
Elspeth
Marija Backovic wrote:
> Dear all -
>
> Does anyone know of a facility that provides amino acid analysis for
> determination of SeMet incorporation in a recombinant protein?
>
> I contacted centers at Grenoble and Uppsala, but SeMet determination
> is not something they routinely do.
>
> Any suggestions would be highly appreciated!
>
> Best,
>
> Marija
>
>
> Marija Backovic, Ph.D.
>
> Pasteur Institute
> Department of Virology
> 25 rue du Dr Roux
> 75015 Paris
> France
>
> +33 (0)1 45 68 82 87
--
******NOTE NEW E-MAIL ADDRESS AND PHONE NUMBER************
----------------------------------------------------
Elspeth F. Garman,
President, British Crystallographic Association
Professor of Molecular Biophysics, University of Oxford
Visiting Professor in Chemistry, University of Durham
Postal address:
Laboratory of Molecular Biophysics,
Department of Biochemistry,
University of Oxford, Tel: (44)-1865-613297
South Parks Road, FAX: (44)-1865-613201
OXFORD, OX1 3QU, U.K. E-mail: [log in to unmask]
http://www.biop.ox.ac.uk/www/garman/gindex.html
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