or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
Good luck
EFG
Paul Leonard wrote:
> Hi Claudia
>
> It really is best to test the cryo condition in an X-ray beam.
>
> However, if you don't have access to X-rays in house you could look at the
> hampton crystallization screen conditions (or another supplier). Find a
> condition with a similar amount and type of precipitant as you have in
> your crystallisation condition and look up how much glycerol they added as
> cryoprotectant. Then try making up your own crystallisation condition
> with the amount of glycerol they recommend and freeze your crystals in
> that solution.
>
> Also try crystal soaks of varying lengths of time (30 seconds - 5 min) in
> the cryo condition as well as trying to soak some crystals where you
> gradually increase the %glycerol in the crystal soak prior to freezing (in
> 2% glycerol steps for example).
>
> see
> http://hamptonresearch.com/product_detail.aspx?cid=1&sid=20&pid=3
>
> Good luck.
>
> Paul
>
>>
>> Dear List,
>>
>>
>>
>> Sorry for the probably silly question.
>>
>>
>>
>> Any suggestions to test cryoconditions without X-rays or cryostream?
>>
>>
>>
>> I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is
>> it enough to try to freeze the cryoconditions in liquid nitrogen checking
>> under the microscope (or by eye) or is this still risky?
>>
>>
>>
>> Thanks,
>>
>>
>>
>> Claudia
>>
>>
>>
>>
>>
>> Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia
>> Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel.
>> 0039 0382 986335/8/1 Facs 0039 0382 303673
>>
>>
>>
>> _________________________________________________________________
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>
>
> Department of Biochemistry
> UMDNJ-Robert Wood Johnson Medical School
> Center for Advanced Biotechnology and Medicine
> 679 Hoes Lane
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>
> Phone: (732) 235-4206
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>
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