PISA gives you quite a lot of information on hydrophobic interactions,
down to atomic level (subject to interpretation). In the list of
interfaces, PISA gives just the sum hydrophobic effect for each interface.
If you click on interface number, it will take you to further details
for the interface, which includes list of residues and their hydrophobic
contributions. Read on-line documentation if in doubt. Bear in mind that
almost all lines in PISA output are hyperlinks to documentation pages,
even if they are not highlighted as links.
On Wed, 19 Aug 2009, Haitao ZHANG wrote:
> Hi, everybody
> I have a crystal structure as homodimer, from which residues in the dimer
> interface need to be identified. Using PISA on the EBI website,
> residues involved in forming hydrogen bonds, salt bridges, disulfide bonds
> and covalent bonds could be identified. But hydrophobic interaction could
> also play an essential role on the dimer stablization. So does anybody know:
> 1. how to find those hydrophobic residues (software or on-line-server)
> 2. how to confirm that whether they play a role in the dimerization, except
> for site-mutation.
> Thanks a lot!