Hi Sebastiano,
Have a look at HATODAS:
http://hatodas.harima.riken.go.jp/
Good luck,
Miguel
Le 15 juil. 09 à 14:33, Sebastiano Pasqualato a écrit :
> Hi all,
> I've got crystals of a protein of ca 200 residues, with 2 free
> cysteines, 5 histidines, 2 methionines.
> We have nice diffraction for the native crystals, that grow in 150
> mM KSCN, 17% PEG 3350, bis tris propane pH 8.8.
> We are crystallising the SeMet derivative, but I'm not completely
> sure I will be able to have nice crystals by saturday, when we have
> tunable time at the ESRF.
> I was thinking of trying with some heavy atom soaks, but only have
> like 30 crystals, so limited trials allowed!
> Which compound would you advice as more likely to work, and thus
> worth testing?
> Thanks in advance for the suggestions,
> ciao
> s
>
>
>
> --
> Sebastiano Pasqualato, PhD
> IFOM-IEO Campus
> Dipartimento di Oncologia Sperimentale
> Istituto Europeo di Oncologia
> via Adamello, 16
> 20139 - Milano
> Italy
>
> tel +39 02 9437 5094
>
> --
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--
Miguel Ortiz Lombardía
Architecture et Fonction des Macromolécules Biologiques (UMR6098)
CNRS, Universités d'Aix-Marseille I & II
Case 932
163 Avenue de Luminy
13288 Marseille cedex 9
France
Tel : +33(0) 491 82 55 93
Fax: +33(0) 491 26 67 20
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Web: http://www.pangea.org/mol/spip.php?rubrique2
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