Good question!
________________________________
From: CcpNmr software mailing list on behalf of Ruth Mirams
Sent: Thu 02/07/2009 15:57
To: [log in to unmask]
Subject: Tracking shift changes in protein mutants
Hi everyone,
I am looking for some help comparing shift lists. I have recorded a series
of HSQC spectra for different mutants of the same protein, and I would like
to analyse the changes in chemical shift that these mutations cause.
Firstly I tried to do this using the "shift differences" popup in the data
analysis tab, but I could not get the shift list comparison for the two
spectra to display (however the shift list comparisons would display to
compare the HSQC for the WT and the triple res data for the WT). I then
tried to make an NMR series (to follow these changes with the "follow shift
changes" popup), but this doesn't seem to work for me either, because in the
condition variable (in the NMR series popup) there is no option for such
mutants as the variable.
Does anyone know the best way to get a shift list comparison and/or
histograms to compare my mutants?
Thanks so much for your help,
Ruth
|