Hi Bill,
5-10% glycerol usually helps in the majority of cases. However, some
proteins require a scan of different conditions and different protein
concentrations. Like everything else, it's protein dependent.
Changing the wavelength can also make a difference. I also know some
SAXS beamlines like at the APS have capillary flow cells so they just
do hit and runs.
Susan
On Jul 9, 2009, at 4:45 PM, William Scott wrote:
> Hi folks:
>
> A colleague of mine here is doing small angle X-ray scattering at
> SSRL and finding his samples are suffering significant radiation
> damage. He asked me for advice as to what potential radical
> scavengers might be useful. I told him to take 10 mg of ascorbic
> acid and to call me in the morning.
>
> Anyone have a better suggestion that I might be able to pass along
> to him?
>
> Many thanks in advance.
>
> Bill
>
>
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