Perhaps the bubbles are related to the bubbles which form when mixing
alcohols with water, e.g., when making coomassie destain? And what are those
bubbles, by the way?
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
----- Original Message -----
From: <[log in to unmask]>
To: <[log in to unmask]>
Sent: Monday, July 06, 2009 5:56 PM
Subject: Re: [ccp4bb] Bubbles on crystals
> Hi,
>
> If you're worried - instead of transfering the crystal, just flood the
> entire drop with excess of your cryo buffer. No exposure to air this way.
> I bet that the bubbles won't go away though :)
>
> Artem
>
>> Dear members,
>> I have protein crystals grown in a condition with Isopropanol (13%) and
>> citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
>> glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
>> Isopropanol), small bubbles appear on the surface of the crystals. These
>> tiny bubbles sometimes disappear in few seconds and sometimes not.
>> Crystals
>> are exposed to air only for 2-3 seconds in the process of transfer. I am
>> worried if the formation of these bubbles is due to evaporation of
>> isopropanol form the crystal and if it affects the crystal lattice. These
>> crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this
>> problem?
>> Thank you
>> Kumar
>>
>
|