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Subject:
Re: How to express a >95KD FAD protein
From:
Kn Ly <[log in to unmask]>
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Date:
Sat, 4 Jul 2009 06:30:59 +0100
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text/plain
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text/plain (6 lines)
try codon optimisation use Rosetta 2 strains fuse to MBP I expressed a yeast protein of ~100 KDa, expression was low until I fused it to MBp (resulting protein ~ 150 KDa)
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