Krisztina,
if you are using the Match Peaks option, then you are only able to match
to spectra which are shown in the window from which you are doing the
matching. So make sure that your CBCACONH and/or HNCOCA are 'switched
on' and shown in the same window as your HNCA when you do the matching.
Tim, Wayne,
perhaps this is something that could be changed? I have often wondered
why you are only able to match in spectra which are 'switched on' in the
query window - would it make more sense to allow matching to any
spectrum with the correct dimensions?
Vicky
Krisztina Feher wrote:
> Dear All,
>
> I was using the backbone assignment procedure suggested on
> http://www.protein-nmr.org.uk/assignment_practice.html. At stage
> Linking sequential Spin Systems, section 5 I got stuck. When I want
> to match the CA shift from the HNCA (I do not have HNCACB) the
> Peak/MatchPeaks menu only offers searching in the HNCA or in active
> spectra (which is thesame), but not in the CBCACONH or HNCOCA. Any one
> have an idea what is happening?
>
> Thanks in advance,
> Krisztina
>
>
>
>
>
>
> --- On *Mon, 5/25/09, Wayne Boucher /<[log in to unmask]>/* wrote:
>
>
> From: Wayne Boucher <[log in to unmask]>
> Subject: Re: sidechain NHs in HSQC
> To: [log in to unmask]
> Date: Monday, May 25, 2009, 4:14 PM
>
> Hello,
>
> If you go to Windows --> Axes into the Axis Types dialog then the
> sixth
> (Region) column contains the defaults for the isotope "universe".
> So just
> edit that and hopefully it will do what you want.
>
> Wayne
>
> On Mon, 25 May 2009, Krisztina Feher wrote:
>
> > Hi,
> >
> > I have recorded a unfolded 15N HSQC between 80ppm and 260ppm to see
> > sidechain NHs. When I open the spectrum in ccpn I only get half
> of it
> > between 80ppm and 160ppm while in TOPSPIN I got the full
> sweepwidth. What
> > should I do to see the other half too?
> >
> > Thanks in advance,
> > Krisztina
> >
>
>
--
*********************************************************
Dr. Victoria A. Higman
Leibniz-Institut fuer Molekulare Pharmakologie (FMP)
NMR-Supported Structural Biology
Robert-Roessle-Str. 10
13125 Berlin
Germany
and
School of Chemistry
University of Bristol
Bristol BS8 1TS
United Kingdom
Phone: +44-117-95-46325
E-mail: [log in to unmask] (or [log in to unmask])
http://www.protein-nmr.org.uk
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