Andy,
One important thing if your complex crystals are isomorphous with apo is to
use nFo(complex) - Fo(apo). These maps give the maximum information as the
uninterpretable 'stuff' in the Fo-Fc map is likely quite similar in both
crystal forms so the difference signal should be cleaner.
Douglas H. Ohlendorf Phone:
612-624-8436
Professor FAX:
612-624-5121
Dept. of Biochemistry, Molecular Biology & Biophysics
Twin Cities Campus, University of Minnesota
Lab web site:
http://biosci.cbs.umn.edu/bmbb/ohlen_lab/index.html
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of ANDY
DODDS
Sent: Wednesday, June 17, 2009 8:30 AM
To: [log in to unmask]
Subject: [ccp4bb] Difference Map images
Hello,
I was wondering what people used to generate difference map images of,
say, a ligand in their structures?
e.g. Figure 2a here
http://journals.iucr.org/f/issues/2009/05/00/tt5012/tt5012.pdf
Cheers,
Andy
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