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ACB-CLIN-CHEM-GEN  May 2009

ACB-CLIN-CHEM-GEN May 2009

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Subject:

Re: Stability of Chromium and Cobalt

From:

Liju Yang <[log in to unmask]>

Reply-To:

Liju Yang <[log in to unmask]>

Date:

Fri, 29 May 2009 13:55:32 -0400

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (143 lines)

Although the metals would not degrade, the metal concentrations may
change after long term storage because of absorption to and release from
tube or stopper. We presume the stability of metal concentrations is not
an issue provided the samples are kelp frozen (metals would stop
moving), but do not have a reference. 

We measure chromium and cobalt concentrations in red cells to evaluate
the wear of orthopaedic implants. As Barry has mentioned, it is easier
and safer to use whole blood as the sample of choice for external blood
draw centre. Separation of red cells from plasma is little bit
complicated and may introduce some errors if not done appropriately, eg,
contamination from pipette or dilution by plasma is not removed
completely. If you are to measure the metals in red cells, you should
provide the clients with the detailed collection procedure.

Generally, we use whole blood for toxic metals such as Lead and Mercury
and red cells for essential elements, including Chromium, Cobalt,
Copper, Manganese, Molybdenum, Selenium, Vanadium and Zinc.  I found
most of trace elements labs measure these elements in whole blood. Just
wonder anyone else also uses red cells for these elements? 
Thanks and have a wonderful weekend.
Liju 


Liju Yang, PhD, FCACB
Clinical Chemist
Clinical Immunology/Trace Elements Laboratory
University Hospital
London Health Sciences Centre
London, ON N6A 5A5
Tel. (519) 685 8500 ext. 35768
Fax. (519) 663 3980


>>> Reza Morovat <[log in to unmask]> 2009/05/28 7:36 PM >>>

Dear Liju,

If you can find a way to destroy them or convert them to something
else, pollution control agencies would love to hear from you!  ;o) 
Incidentally, do you measure them in serum, as some say is a better
indicator of the load (though mostly ??trivalent and benign), or
specifically in red cells, as some say is a better indicator of the
toxic fraction?  Could you or someone advise me on this please?

Thanks,

Reza


 

Reza Morovat

Clinical Biochemist

Oxford

 
> Date: Wed, 27 May 2009 14:58:26 -0400
> From: [log in to unmask] 
> Subject: Stability of Chromium and Cobalt
> To: [log in to unmask] 
> 
> Dear All,
> 
> Does anyone have information on stability of trace elements Chromium
and Cobalt in frozen blood (whole blood or red blood cells) samples on
BD heparin or EDTA tubes? 
> 
> Thanks 
> 
> 
> 
> Liju Yang, PhD, FCACB
> Clinical Chemist
> Clinical Immunology/Trace Elements Laboratory
> University Hospital
> London Health Sciences Centre
> London, ON N6A 5A5
> Tel. (519) 685 8500 ext. 35768
> Fax. (519) 663 3980
> 
> 
>
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Please note, archived messages are public and can be viewed
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ACB Web Site
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